9-66
FROM: Standard Methods for the Examination of Water and Wastewater
9222 F. KIebsieIIa Membrane Filter Proc
Klebsiclla bacteria belong to the family Enterobacteriaceae and
are included in the total coliform group. The outermost layer of
Klebsiella bacteria consists of a large polysaccharide capsule, a
characteristic that distinguishes this genus from most other bac-
teria in this fami1y: this capsule provides some measure of pro-
tection from disinfectants. Klebsiella bacteria are commonly as-
sociated with coliforrn regrowth in large water supply distribution
systems.
Klebsiellae may he opportunistic pathogens that can give rise
to bacteremia, pneumonia, urinary tract, and several other types
of human infection. Approximately 60 to 80% of all Klebsiella
from feces and from clinical specimens are positive in the fecal
coliform test and are Klebsiella pneumoniae.
Klebsiella bacteria also are widely distributed in nature, occur-
ring in soil, water, grain, vegetation, etc. Wood pulp, paper mills,
textile finishing plants, and sugar-cane processing operations con-
tain large numbers of klebsiellae in their effluents (104 to 106),
and Klebsiella sp. are often the predominant coliform in such effluents
Rapid quantitation may be achieved in the MF procedure by
modifying M-FC agar base through substitution of inositol for
lactose and adding carbenicillin or by using M-Kleb agar. These
methods reduce the necessity for biochemical testing of pure
strains. Preliminary verification of differentiated colonies is rec-
ommended.
1. Apparatus
a. Culture dishes: See Section 9222B.le for specifications,
b. Filtration units: See Section 9222B.lf
2. Materials and Culture Medium
a. Modified M-FC agar (M-FC[C agar): This
he available in dehydrated form and may require
the basic ingredients: